ABSTRACT
PURPOSE: This study analyzed the effectiveness of poly (lactic-co-glycolic acid) (PLGA) as a tissue recovery agent and determines the in vivo safety and efficacy of microparticle-based PLGA. MATERIALS AND METHODS: Fifteen 3-month-old male white rabbits were used. Allogenic adipose tissue derived stromal vascular fraction (SVF) was cultured and labeled with the fluorescent dye PKH26. The rabbits were divided into 4 groups: the SVF group, the PLGA group, the normal control group, and the disease control group. The right corpus cavernosal tissue of the rabbits was surgically removed in the selected portion, except in the normal control group. The defect space of each rabbit was replaced with 10(6) SVF cells in the SVF group and 0.1 g of biodegradable polymer solution in the PLGA group. Microscopic confirmation and analysis of tissue regeneration were performed after 8 weeks. Using confocal microscopy, the nuclei of the smooth muscle cells and SVF migration were examined. The composition of smooth muscle and fibrosis of the injured corpus cavernosum were compared and analyzed by Masson's trichrome stain. RESULTS: There were no signs of migration or rejection of the injected materials in any of the experimental groups. The mean amount of smooth muscle in the normal control group was 15.25+/-1.34 microm2 (right) and 13.90+/-0.703 microm2 (left); in the disease control group it was 11.10+/-0.87 microm2 (right) and 12.80+/-1.01 microm2 (left); in the SVF group it was 13.82+/-4.10 microm2 (right) and 13.96+/-3.94 microm2 (left); and in the PLGA group it was 12.89+/-1.39 microm2 (right) and 13.24+/-1.43 microm2 (left). Only the disease control group showed significant decreased smooth muscle in the left cavernosum (p0.05). Furthermore, no difference was found between any two groups (normal control versus SVF (p=0.705), normal control versus PLGA (p=0.88), SVF versus PLGA (p=0.23). CONCLUSIONS: PLGA microparticles had the same tissue restoring effect when compared with SVF and no adverse effect or migration of particles was found through the injection of PLGA or SVF. PLGA is safe and has the proper tissue recovery effect, saving additional tissue harvesting.
Subject(s)
Humans , Infant , Male , Rabbits , Adipose Tissue , Fibrosis , Lactic Acid , Microscopy, Confocal , Muscle, Smooth , Myocytes, Smooth Muscle , Organic Chemicals , Penis , Polyglycolic Acid , Polymers , Regeneration , Rejection, Psychology , Tissue and Organ HarvestingABSTRACT
A clinical observation on the nephrectomized 114 patients in the department of Urology, Han Gang Sung Sim Hospital, during the period from January, 1972 to December, 1979 was presented with brief review of the literature. The result were as follows: 1. The total number of in-patients during above period was estimated to 1492. and nephrectomies were performed in the 114 of the 913 urologic operations. (12.4%) 2. The age distribution ranged from 1 year to 66 years with the most common age groups in the 4 th decades. (22.8%) 3. The ratio of male to female was 1.2 to 1. 4. Among the symptoms on admission, hematuria was in 82 cases (71.9%), flank pain in 74 cases (64.9%), and urinary frequency in 46 cases. (40.4%) 5. The causative diseases of the operated kidney showed the highest in renal tuberculosis (30.7%), renal tumor (18.4%), calculous disease (17.5%). renal injury (14.9%) and hydronephrosis (10.5%) by sequence. 6. Flank approach with 1 rib resection was the most frequently used in 52 of 114 cases. 7. Postoperative complications were seen with 3 cases of wound infection, 2 cases of wound dehiscence, 2 cases of atelectasis, 1 case of pneumonia, and 1 case of paralytic ileus. 8. 110 cases (96%) were discharged within 2 weeks after surgery.
Subject(s)
Female , Humans , Male , Age Distribution , Flank Pain , Hematuria , Hydronephrosis , Intestinal Pseudo-Obstruction , Kidney , Nephrectomy , Pneumonia , Postoperative Complications , Pulmonary Atelectasis , Ribs , Tuberculosis , Tuberculosis, Renal , Urology , Wound Infection , Wounds and InjuriesABSTRACT
Recently we experienced 10 cases of posterior urethral injury associated with pelvic fracture. Of these patients 5 underwent primary realignment with perineal traction on the prostate (Selikowitz method), and the other 5 underwent immediate cystostomy and delayed urethroplasty (Johanson method). Comparison of the post-operative complications didn`t appear to show significant differences in the 2 groups, although the numbers were small. But patients who performed immediate cystostomy and delayed urethroplasty showed some disadvantages of the long period of treatment, high charges, often multiple hard procedures and the long time of operation. Therefore in the initial management of posterior urethral injury, we think it better to take primary realignment in Korean socioeconomic circumstances, if the patient is not in the high risk.